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SpinStarTM Extraction Kits
SpinStarTM Viral Nucleic Acid Extraction Kit
The SpinStarTM Viral Nucleic Acid Kit is intended for simultaneous purification of viral DNA and RNA from fresh or frozen human serum and plasma, respiratory specimens in VTM and stool. Nucleic acid extraction is based on proven silica membrane technology in spin column format. The purified viral DNA and/or RNA is ready to use for downstream analysis. The SpinStarTM Viral Nucleic Acid Kit can be used for isolation of viral RNA and DNA from a broad range of RNA and DNA viruses.
SpinStarTM Total DNA Extraction Kit
The SpinStarTM Total DNA Kit is intended for purification of total DNA (e.g. genomic, mitochondrial and of bacterial, viral and parasitic origin) from up to 400 μl human whole blood, serum, plasma, respiratory specimen in VTM and up to 200 μl sputum (pretreated) or stool suspension. Nucleic acid extraction is based on proven silica membrane technology in spin column format. The purified DNA is ready to use for downstream analysis, for example real-time PCR.
SpinStarTM Pretreatment Solution
For ultrasensitive extraction of viral nucleic acids from human serum and plasma.
The SpinStarTM Pretreatment Solution allows concentration of viral DNA and RNA from 1 ml plasma or serum, prior to purification with the SpinStarTM Viral Nucleic Acid Kit. Ultracentrifugation and specialized laboratory equipment are not required.
The Pretreatment Solution forms complexes with nucleic acids, which can be sedimented by centrifugation to form a pellet. This pellet is then resuspended in standard volume of lysis buffer and nucleic acids are subsequently purified using the SpinStarTM Viral Nucleic Acid procedure, enabling efficient and ultrasensitive viral nucleic acid extraction.
The SpinStarTM procedure comprises 4 steps (lyse, bind, wash, elute) and is carried out using SpinStarTM columns in a standard microcentrifuge. The procedure is designed to ensure that there is no sample-to-sample cross-contamination and allows safe handling of potentially infectious samples. The simple SpinStarTM procedure, which is highly suited for simultaneous processing of multiple samples, yields pure nucleic acid in approx. 1 hour.